Subtilisin
Detailed Description
Product name:Subtilisin
CAS:9014-01-1
Description:
Subtilisin is a non-specific protease (a protein-digesting enzyme) initially obtained from Bacillus subtilis.
Subtilisins belong to subtilases, a group of serine proteases that - like all serine proteases - initiate the nucleophilic attack on the peptide (amide) bond through a serine residue at the active site. Subtilisins typically have molecular weights of about 20,000 to 45,000 dalton. They can be obtained from certain types of soil bacteria, for example, Bacillus amyloliquefaciens from which they are secreted in large amounts.
Applications:
(1)Research tool
In molecular biology using B. subtilis as a model organism, the gene encoding subtilisin (aprE) is often the second gene of choice after amyE for integrating reporter constructs into, due to its dispensability.
(2)Commercial
Protein-engineered subtilisins are widely used in commercial products (the native enzyme is easily inactivated by detergents and high temperatures) and is also called a stain cutter, for example, in laundry and dishwashing detergents, cosmetics, food processing,skin care ointments,contact lens cleaners, and for research in synthetic organic chemistry.
- Subtilisin
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