recombinant human trypsin
Detailed Description
Animal Origin Free, No Virus Contaminant
DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. Recombinant Human Trypsin is a genetically engineered protein, expressed in E.Coli and purified by high pressure liquid chromatography. The amino acid sequence is the same as Human Trypsin 2 sequence. The pH optimum of trypsin is 7 - 9. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA. There are no contaminating enzyme activities such as from carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
ADVANTAGES
Animal origin free
The use of recombinant Human Trypsin eliminates the risk of virus presence, and of any other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Human Trypsin belongs to the AOF level 3.
Recombinant human trypsin
The amino acid sequence is the same as the Human Trypsin 2 sequence.
Stable
A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity
1)Recombinant human trypsin provides increased specificity and eliminates contaminating activities found in lower purity enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
3) One single main band at 24kD by SDS-PAGE.
Source E. Coli
Purified by HPLC
Physical form White or White-like lyophilized powder
Additives NaCl salts and carbohydrates.
Protein content 35% ~ 50%
Specific activity >3000 USP units/mg pro.
Purity > 95%
Contaminant activity No chymotrypsin, carboxypeptidase A, and other protease contaminant.
- recombinant human trypsin
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