Title: Neotetrazolium Chloride
CAS Registry Number: 298-95-3
CAS Name: 3,3¢-[1,1¢-Biphenyl]-4,4¢-diylbis[2,5-diphenyl-2
H-tetrazolium] dichloride
Synonyms: 3,3¢-[4,4¢-biphenylene]bis[2,5-diphenyl-2
H-tetrazolium] dichloride; 2,2¢,5,5¢-tetraphenyl-3,3¢-(
p-diphenylene)ditetrazolium chloride; 2,2¢-(
p-diphenylene)-bis[3,5-diphenyl]ditetrazolium chloride; neotetrazolium blue; neo-T; TP; NTC
Molecular Formula: C38H28Cl2N8
Molecular Weight: 667.59
Percent Composition: C 68.37%, H 4.23%, Cl 10.62%, N 16.78%
Literature References: Prepn: L. J. Pannone, J. B. Rust,
US 2713581 (1955 to Montclair Res. Corp. and Ellis-Foster Co.). Purification: G. R. N. Jones,
Histochem. J. 1, 59 (1968).
See also Triphenyltetrazolium chloride.
Properties: Light yellow powder, mp 297° (dec).
Melting point: mp 297° (dec)
Use: Staining of microorganisms and plant and animal tissues. In determn of dehydrogenases in histochemical and cytochemical studies: K. Neumann, G. Koch,
Z. Physiol. Chem. 295, 35 (1953). In amylase determn.