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CAS No 96314-98-6 , fura 2

  • Name: fura 2
  • Synonyms: 2-[6-[Bis(carboxymethyl)amino]-5-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid;1-[6-Amino-2-(5-carboxy-2-oxazolyl)-5-benzofuranyloxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraaceticacid,pentapotassiumsalt;fura 2;FURA 2;1-(2-[5-CARBOXYOXAZOL-2-YL]-6-AMINOBENZOFURAN-5-OXY)-2-(2'-AMINO-5'-METHYLPHENOXY)ETHANE-N,N,N',N'-TETRAACETIC ACID;
  • CAS Registry Number:
  • Flash Point: 548.6°C
  • Boiling Point: 983.5°C at 760 mmHg
  • Density: 1.58g/cm3
  • Refractive index: 1.684
  • Flash Point: 548.6°C
  • Molecular Weight: 641.54
  • InChI: InChI=1S/C29H27N3O14/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42)
  • Molecular Formula: C29H27N3O14
  • Molecular Structure:CAS No:96314-98-6 fura 2

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96314-98-6 fura 2

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References of fura 2
Title: Fura-2
CAS Registry Number: 96314-98-6
CAS Name: 2-[6-[Bis(carboxymethyl)amino]-5-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid
Molecular Formula: C29H27N3O14
Molecular Weight: 641.54
Percent Composition: C 54.29%, H 4.24%, N 6.55%, O 34.91%
Literature References: Fluorescent calcium imaging agent; structurally related to BAPTA, q.v. Ca2+ binding causes a spectral shift to shorter wavelengths; ratio of the fluorescence at each wavelength is related to changes in Ca2+ binding and is independent of dye concentration. Also used as pentaacetoxymethyl ester, Fura-2/AM, to enhance crossing of membranes. Prepn: G. Grynkiewicz et al., J. Biol. Chem. 260, 3440 (1985). Binding kinetics to Ca2+: A. P. Jackson et al., FEBS Lett. 216, 35 (1987). Determn of Kd: D. L. Groden et al., Cell Calcium 12, 279 (1991). Spectra of intracellular forms: C. S. Owen, ibid. 385. Photophysics: V. Van den Bergh et al., Biophys. J. 68, 1110 (1995). HPLC determn: M. Castle, E. Neuteboom, J. Chromatogr. A 696, 93 (1995). Determn in tissue: N. N. P. Tran et al., Cell Calcium 18, 420 (1995). Practical aspects of use in intracellular Ca2+ measurement: K. R. Sipido, G. Callewaert, Cardiovasc. Res. 29, 717 (1995).
Properties: uv max absorption (100 mM KCl): 362 nm free dye; 335 nm Ca2+ complex. emission max: 512 nm free dye; 505 nm Ca2+ complex.
Absorption maximum: uv max absorption (100 mM KCl): 362 nm free dye; 335 nm Ca2+ complex
Use: Fluorescent probe for measurement of calcium in biological systems.