Title: Deoxyribonuclease I
CAS Registry Number: 9003-98-9
Synonyms: Deoxyribonuclease (pancreatic); pancreatic dornase; pancreatic desoxyribonuclease; DNase I
Literature References: Endonuclease enzyme that hydrolyzes DNA; produced by the mammalian pancreas. Variably glycosylated, single polypeptide chain with two disulfide bridges; multiple isoforms exist. The human form consists of 260 amino acid residues; mol wt ~37 kDa. Prepn from beef pancreas: M. Kunitz,
Science 108, 19 (1948); Baumgarten, Johnson,
US 2801956;
eidem, US 3042587 (1957, 1962 both to Merck & Co.). Properties of purified DNase I: Price
et al., J. Biol. Chem. 244, 917 (1969). Reviews of purification and characterization: M. Laskowski in
The Enzymes vol. 4, P. D. Boyer, Ed. (Academic Press, New York, 3rd ed., 1971) pp 289-311; S. Moore,
ibid. vol. 14 281-296 (1981); T.-H. Liao,
Mol. Cell. Biochem. 34, 15-22 (1981).
Derivative Type: Bovine pancreatic deoxyribonuclease
Trademarks: Deanase (Consolidated Chem.); Dinase (Eufarma); Dornavac (Merck & Co.)
Properties: Mixture of 4 isoforms. DNAse A is the most predominant. Contains 257 amino acid residues; mol wt 31 kDa. pI ~5.
Derivative Type: Dornase alfa
CAS Registry Number: 143831-71-4
CAS Name: Deoxyribonuclease (human clone 18-1 protein moiety)
Synonyms: rhDNase I
Trademarks: Pulmozyme (Genentech)
Literature References: Human DNase I produced in Chinese hamster ovary cells by recombinant DNA technology. Cloning and expression of human form and effect on sputum viscosity: S. Shak
et al., Proc. Natl. Acad. Sci. USA 87, 9188 (1990); S. Shak,
WO 9007572 (1990 to Genentech). HPLC purification: J. Cacia
et al., J. Chromatogr. 634, 229 (1993). Clinical study in cystic fibrosis: H. J. Fuchs
et al., N. Engl. J. Med. 331, 637 (1994). Expert report on pharmacology and toxicology: J. D. Green,
Hum. Exp. Toxicol. 13, Suppl. 1, S1-S42 (1994).
Therap-Cat: Debriding agent. In treatment of cystic fibrosis and other pulmonary disease to reduce viscosity of sputum.
Therap-Cat-Vet: Debriding agent.
Keywords: Debriding Agent.
